Anesthesia Research/Research Assistant II/Day/40 Hours/Regular
Our researchgroup is looking for a responsible, highly motivated, enthusiastic individualto join us as a Laboratory Research Technician. We conduct both basic andtranslational research on the neurobiology of cognitive impairment after generalanesthesia and surgery. In the laboratory, we use rodent models to examine howgeneral anesthesia and surgery affect the brain, with current emphasis on gliaand neuroinflammation. To that end, we use methods that include fluorescenceand magnetic activated cell sorting, RNA isolation and purification, PCR, immunocyto-and histochemistry, cell culture, and animal behavior. Working under thegeneral supervision of the Principal Investigators, this individual will developexpertise in several methods, conduct in vivo experiments in rodents, performin vitro experiments in rodent brain cells and slices, and compile and analyzedata using basic statistics.
PRINCIPAL DUTIES ANDRESPONSIBILITIES:
Executeexperiments using established methodologies. Techniques currently in useinclude:
Anesthesia,surgery, and physiological monitoring of rodents
Braintissue harvesting and dissection
Cellsorting (FACS, magnetic)
ELISA,RNA isolation amp; purification, cDNA preparation, PCR
Maintainand analyze primary cell cultures – sterile technique required
Cellfixation and staining for image analysis
Fluorescencemicroscopy, including high-throughput and confocal imaging
Animalbehavior - analysis of memory (water and radial arm mazes)
Record data, summarize, and analyzeresults using basic statistics.
Prepare and maintain laboratoryreagents, chemicals, instruments, and equipment.
Maintain supplies inventory inaccordance with departmental procedures.
Maintaina transgenic mouse colony.
Manageaccounts of multiple grants.
Manageand maintain animal care approvals
Discussprogress of work regularly with supervisor.
Allother duties as assigned.
The idealcandidate will have a BA/BS in Neuroscience, Biology, or a related sciencefield and an interest in medicine and medical research. A willingness to workwith rodents is required. Previous laboratory experience and know-how, eitherwork-related or academic, is strongly preferred, though job-specific techniqueswill be taught as needed. We most desire an individual who is responsible,organized, self-motivated, curious, and able to multi-task. She/he must also beable to work well independently and as part of a team. A two-year commitment isrequired.
If youhave these qualities and the opportunity sounds interesting, please send an emailand statement of interest, a resume (including GPA), and a list of threereferences.
Supervise students who join the lab as summerinterns.
Brigham and Women's Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, creed, sex, sexual orientation, gender identity, national origin, ancestry, age, veteran status, disability unrelated to job requirements, genetic information, military service, or other protected status.
Primary Location MA-Boston-BWH Boston Main Campus
BWH Boston Main Campus (BWHMAIN)
75 Francis St